Download Advances in Lipid Methodology. Volume 3 by W. W. Christie PDF

By W. W. Christie

This is often the 3rd quantity of an occasional sequence of evaluate volumes facing features of lipid technique. As with the 1st volumes, issues were chosen which were constructing speedily lately and feature a few significance to lipid research. The authors are all major overseas experts.

Topics lined contain: research of positional isomers of glycerolipids via non-enzymatic equipment, separation of phospholipid sessions via high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid section behaviour and lipid diffusion, between others

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Extra info for Advances in Lipid Methodology. Volume 3

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1 Hz under optimal conditions. 01-100 mg) is dissolved in 2 ml reagent A and transferred to a 10 mm NMR sample tube; 1 ml reagent B is added, and the combination mixed thoroughly. A small aqueous phase separates and is allowed to rise to the top of the sample (1 min). Ordinarily, there is no need to remove this small aqueous phase, which contains no detectable phospholipids, and its presence above the sample ensures that the proper equilibria are maintained and that the organic solvents will not evaporate significantly during the NMR analysis.

10. ) column of Sumipax OA-4100. However, the peaks tailed rather badly and the addition of one double bond or two carbons to an enantiomeric homologue resulted in an overlapping pair. Thus, sn-1-16:0 and sn-1-18:1 were not resolved and nor were the corresponding enantiomers. A preliminary silver ion TLC fractionation on boric acid-treated plates was necessary to overcome this problem. The Sumipax OA-4100 liquid phase is also suitable for the separation of natural mixtures or enantiomeric monoacylglycerols, as demonstrated by Takagi and Ando [97].

The aqueous layer is usually EDTA-saturated, and, depending on the nature and quantity of the sample lipid, a third phase, precipitated EDTA, may appear within the aqueous phase. It is the aqueous-phase EDTA that contains the alkaline-earth and transition-metal cations purged from the sample, in addition to the bulk of the Cs [14,54]. Small amounts of EDTA, however, always remains within the chloroform phase [54]. 1 shows a 31 P NMR phospholipid profile obtained from a fresh water sponge using the described reagent.

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